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Non-invasive approaches include the detection of biological markers such as enzymes, antibodies, or specific proteins (e.g., lactoferrin, calprotectin, and C-reactive protein in bodily fluids that can be collected non-invasively, such as urine, fecal matter, or saliva (Hickman, 2024

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One of the concerning additives that has been detected in some liquids is diacetyl, a flavor enhancer that has been linked to lung damage when inhaled

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More commonly, addressing underlying causes of deficiency (malabsorption, inadequate dietary intake, increased losses) and optimising dietary zinc intake proves sufficient